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11.
A Alam 《Analytical biochemistry》1992,203(1):121-126
The principle of the protein assay using the reaction of an alkaline copper-protein complex with the Folin-Ciocalteu phenol reagent has been investigated. In contrast to the long-established Lowry method, a stable and rapid protein assay is developed without a buffering agent in alkaline copper solution. In the absence of a buffering agent, the reaction pH drops relatively rapidly and moves the reaction toward a more stable pH. When the reaction of alkaline copper-protein complex with Folin-Ciocalteu reagent is started at around pH 11.7, the reaction color absorbance reaches a plateau in approximately 10 min and remains stable to allow a reliable measurement of the absorbance. In the absence of the buffering agent sodium carbonate, the alkaline copper solution is also stable for months. The principle of the protein assay is presented as a model that can be used to formulate protein assays of desired specification. 相似文献
12.
Christopher M. R. Bax Vijai S. Shankar A. S. M. Towhidul Alam Bridget E. Bax Baljit S. Moonga Christopher L. -H. Huang Mone Zaidi Barry R. Rifkin 《Bioscience reports》1993,13(3):169-174
We report the effects of tetracycline analogues on cytosolic Ca2+ transients resulting from application of ionic nickel (Ni2+), a potent surrogate agonist of the osteoclast Ca2+ receptor. Preincubation with minocycline (1 mg/l) or a chemically modified tetracycline, 4-dedimethyl-aminotetracycline (CMT-1) (1 or 10 mg/l), resulted in a significant attenuation of the magnitude of the cytosolic [Ca2+] response to an application of 5 mM-[Ni2+]. Preincubation with doxycycline (1 or 10 mg/l) failed to produce similar results. In addition, application of minocycline alone (0.1–100 mg/l) resulted in a 3.5-fold elevation of cytosolic [Ca2+]. The results suggest a novel action of tetracyclines on the osteoclast Ca2+ receptor. 相似文献
13.
14.
Abstract: The N-methyl-d -aspartate (NMDA) receptor possesses two distinct amino acid recognition sites, one for glutamate and one for glycine, which appear to be allosterically linked. Using rat cortex/hippocampus P2 membranes we have investigated the effect of glutamate recognition site ligands on [3H]glycine (agonist) and (±)4-trans-2-car-boxy-5,7-dichloro-4-[3H]phenylaminocarbonylamino-1,2,3,4-tetrahydroquinoline ([3H]l -689,560; antagonist) binding to the glycine site and the effect of glycine recognition site ligands on l -[3H]glutamate (agonist), dl -3-(2-carboxypiperazin-4-yl)-[3H]propyl-1 -phosphonate ([3H]-CPP; “C-7” antagonist), and cis-4-phosphonomethyl-2-[3H]piperidine carboxylate ([3H]CGS-19755; “C-5” antagonist) binding to the glutamate site. “C-7” glutamate site antagonists partially inhibited [3H]l -689,560 binding but had no effect on [3H]glycine binding, whereas “C-5” antagonists partially inhibited the binding of both radioligands. Glycine, d -serine, and d -cycloserine partially inhibited [3H]CGS-19755 binding but had little effect on l -[3H]-glutamate or [3H]CPP binding, whereas the partial agonists (+)-3-amino-1-hydroxypyrrolid-2-one [(+)-HA-966], 3R-(+)cis-4-methyl-HA-966 (l -687,414), and 1-amino-1-carboxycyclobutane all enhanced [3H]CPP binding but had no effect on [3H]CGS-19755 binding, and (+)-HA-966 and l -687,414 inhibited l -[3H]glutamate binding. The association and dissociation rates of [3H]l -689,560 binding were decreased by CPP and d -2-amino-5-phosphonopentanoic acid (“C-5”). Saturation analysis of [3H]l -689,560 binding carried out at equilibrium showed that CPP had little effect on the affinity or number of [3H]l -689,560 binding sites. These results indicate that complex interactions occur between the glutamate and glycine recognition sites on the NMDA receptor. In addition, mechanisms other than allosterism may underlie some effects, and the possibility of a steric interaction between CPP and [3H]l -689,560 is discussed. 相似文献
15.
Sonia Mannan Jobair Alam K. M. Ashbarul Bari S. M. A. A. Mamun Rehnuma Mehzabin Orin 《Developing world bioethics》2023,23(3):252-259
Informed consent to medical intervention is fundamental in both ethics and law. But in practice it is often not taken seriously in developing countries. This paper provides an appraisal of informed consent practices in Bangladesh. Following a review of the ethical and legal principles of informed consent, it assesses the degree to which doctors adhere to it in Bangladesh. Based on findings of non-compliance, it then investigates the reasons for such non-compliance through an appraisal of informed consent practices in Bangladesh and provides recommendations aimed at improving such practices. The significance of this paper lies in unveiling the interdependence between the ethical and legal traits of informed consent and their ramifications on strengthening the patient-oriented approach of duty to care. 相似文献
16.
Md. Aktar-Uz-Zaman Md. Ariful Islam Md. Shahin Iqbal Md. Jahangir Alam Debashish Sarkar Bander Albogami Ahmed Gaber Akbar Hossain 《Phyton》2023,92(3):883-899
Ensuring food security for the rapidly increasing population and changing climatic scenarios are requisites for
exploiting the genetic divergence of food crops. A study was undertaken to sort out an early maturing chickpea
variety for fitting easily between rice-rice cropping systems in the Eastern Indo-Gangetic Plain of Bangladesh. The
trial was comprised of eight elite lines of chickpea and executed at various localities in Bangladesh from 2014–
15 to 2017–18. The result explored the chickpea genotype, BARI Chola-11 remained superior to the rest of
the elite genotypes for having a short maturity period (100–106 days), and lesser days to 50% flowering (47–
55 days). The same genotype was recorded to have robust vegetative and reproductive yield attributes including
plant height (49–57 cm), podsplant−1 (37–50), and optimum 100 seed weight (19.5–20.6 g). Owing to better yield
attributes, BARI Chola-11 resulted in the maximum seed yield (1200–1500 kg ha−1
) of chickpea and might be
recommended for general adoption in the region for boosting nutritional security status through improved productivity under changing climate. 相似文献
17.
A lipoidal-protein complex has been isolated from rat gastrocnemius tissue which exhibits a highly specific binding capacity for [3H]veratridine. Purification of the complex has been accomplished by a number of chromatographic steps including affinity chromatography in organic solvents utilizing a resin synthesized by oxirane coupling of veratridine to Sephadex LH-20. The purified complex binds veratridine but not tetrodotoxin or a number of cholinergic ligands. Veratridine binding to the complex is inhibited by aconitine but not tetrodotoxin or cholinergic ligands. The complex has both veratridine saturable (KD= 13 μm ) and non-saturable (KD1 Mm ) binding components. Preliminary chemical analysis showed that the complex is a proteoglycolipid with a protein: carbohydrate: phosphorous ratio of 1.5:1.1:1.0. A discussion is presented favoring the identity of the isolated proteoglycoiipid as a portion of the macromolecular complex comprising the axonal sodium action potential ionophore. 相似文献
18.
Characterization of the IS895 family of insertion sequences from the cyanobacterium Anabaena sp. strain PCC 7120. 下载免费PDF全文
J Alam J M Vrba Y Cai J A Martin L J Weislo S E Curtis 《Journal of bacteriology》1991,173(18):5778-5783
A family of repetitive elements from the cyanobacterium Anabaena sp. strain PCC 7120 was identified through the proximity of one element to the psbAI gene. Four members of this seven-member family were isolated and shown to have structures characteristic of bacterial insertion sequences. Each element is approximately 1,200 bp in length, is delimited by a 30-bp inverted repeat, and contains two open reading frames in tandem on the same DNA strand. The four copies differ from each other by small insertions or deletions, some of which alter the open reading frames. By using a system designed to trap insertion elements, one of the elements, denoted IS895, was shown to be mobile. The target site was not duplicated upon insertion of the element. Two other filamentous cyanobacterial strains were also found to contain sequences homologous to IS895. 相似文献
19.
Structural features of methyl-accepting taxis proteins conserved between archaebacteria and eubacteria revealed by antigenic cross-reaction. 总被引:12,自引:5,他引:7 下载免费PDF全文
A number of eubacterial species contain methyl-accepting taxis proteins that are antigenically and thus structurally related to the well-characterized methyl-accepting chemotaxis proteins of Escherichia coli. Recent studies of the archaebacterium Halobacterium halobium have characterized methyl-accepting taxis proteins that in some ways resemble and in other ways differ from the analogous eubacterial proteins. We used immunoblotting with antisera raised to E. coli transducers to probe shared structural features of methyl-accepting proteins from archaebacteria and eubacteria and found substantial antigenic relationships. This implies that the genes for the contemporary methyl-accepting proteins are related through an ancestral gene that existed before the divergence of arachaebacteria and eubacteria. Analysis by immunoblot of mutants of H. halobium defective in taxis revealed that some strains were deficient in covalent modification of methyl-accepting proteins although the proteins themselves were present, while other strains appeared to be missing specific methyl-accepting proteins. 相似文献
20.
Oral administration of spirobishexahydropyrimidine showed an increase in the activity of serum transaminases, lactate dehydrogenase and alkaline phosphatase. Biological half life and other pharmacokinetic parameters showed rapid absorption and slow elimination of the drug. 相似文献